🧪 Batch Tests · Minireactors · Pilot Plants · Continuous Reactors · Biological Treatment

Treatment Treatability Testing

From simple batch tests to complex continuous reactor studies — before you build, test.

Treatability tests in their different forms — laboratory batch tests, minireactors and pilot plants — predict how a Treatment Plant will perform before its construction and investment. They are the most critical step in the design of any industrial effluent treatment plant, and the most frequently skipped.

Biogroup has vast experience in treatability testing and subject matter experts who can help you solve treatment plant design and operational problems — from a simple batch test lasting a few hours to complex continuous reactor studies extending over longer periods and testing multiple scenarios.

+35 years
Treatability expertise
Batch → Pilot
Full scale range
Aerobic · Anaerobic
Both treatment modes
Own lab
Certified & accredited
⚠️ The most common and costly mistake
Declaring the work finished when construction ends
There is a manifest precariousness we record daily in the analysis of projects and treatment plants in operation — the frequent but fundamentally erroneous conception of declaring the work finished once the construction of the effluent treatment plant is complete. A plant built without treatability data is a plant designed on assumptions, not evidence.
✅ The correct approach
Test before you design. Design before you build.
Before designing an Industrial Effluent Treatment Plant, treatability tests must be carried out. The results define the correct HRT, SRT, nutrient requirements, oxygen demand, power density and loading rate — all the parameters that determine whether your plant will actually work as designed.
The fundamental principle most engineers overlook
It's the microorganisms, not the machines
The work of converting dissolved organic material or substrate is carried out by microorganisms — not by machines such as aerators, biodiscs, or mixers.

This seemingly simple observation has profound consequences for treatment plant design. Aerators provide oxygen and mixing — but they cannot compensate for inadequate biomass, insufficient residence times, or the wrong environmental conditions for the microbial communities doing the actual treatment work.

🦠 Biomass is the asset
The microbial community must be established, acclimatized, and maintained at sufficient concentrations. Equipment only creates the conditions for this to happen.
⏱️ Time is non-negotiable
Biological reactions require adequate contact time — hydraulic and solids retention times that match the kinetics of the specific microbial population treating your specific effluent.
🧪 Every effluent is different
Generic design parameters rarely apply. Your effluent's specific composition determines the correct HRT, SRT, nutrient ratio, oxygen demand and toxicity threshold.
Six critical failure modes that treatability testing prevents
Why treatment plants underperform — the most common design errors
These are the failure modes Biogroup identifies most frequently when auditing treatment plants that are not meeting their design effluent quality targets. Every one of them can be predicted and avoided through proper treatability testing.
1
Insufficient aeration time — air but no microorganisms
For aerobic applications using excessively short aeration/biomass residence times (we have seen up to 10 minutes!), you will have air but no active microbial population. Biological treatment requires adequate SRT to maintain biomass. Short HRTs wash out the microorganisms before they can establish.
2
Wrong oxygen demand calculations
Oxygen requirements calculated from generic BOD5 values frequently underestimate the actual demand — particularly when the effluent contains slowly biodegradable compounds, high ammonia loads requiring nitrification, or substances with elevated oxygen equivalents. The result is chronic oxygen limitation.
3
Insufficient power density — mixing failure
Insufficient power densities fail to achieve adequate mixing for the unit process in question — whether the equalization tank, the main reactor, or polishing lagoons. Each process block has its own recommended power density. Low power means poor contact between microorganisms, substrate, nutrients and oxygen — even with adequate aeration installed.
4
Non-uniform feeding — equalization failure
Practically all unit processes require uniform feeding — both hydraulically and in terms of organic load. Irregular charges or an absent or undersized equalization tank cause severe disruption to biological processes, sometimes interrupting them entirely. Biological populations cannot tolerate sudden load spikes.
5
Organic overloading — biological inhibition
Biological populations have their limits. Sending excessively high organic loads to a biological reactor risks paralyzing biological activity. The maximum organic load a system can accept — the threshold above which performance collapses — must be determined through treatability testing, not assumed from literature values.
6
Incomplete characterization — flawed design basis
Designing a treatment plant based exclusively on BOD₅ is a classic error. A plant designed on BOD₅ alone may underperform due to spontaneous nitrification, nutrient deficiencies, inhibitory compounds, or substances not detected in a basic characterization. Full constituent evaluation is non-negotiable before design.
Our treatability testing services — Three scales of investigation
From bench to pilot — the right scale for each stage of your project
We carry out studies ranging from simple batch tests lasting a few hours to complex continuous reactor studies extending over longer periods and testing several different scenarios — all in our certified and accredited laboratory in Rosario, Argentina.
Scale 1
🧪 Batch / Discontinuous Tests
Simple studies of a few hours to a few days. Rapid screening of treatability, biodegradability, inhibition thresholds and nutrient requirements. The fastest and most cost-effective first step.
Typical determinations:
• BOD kinetics · ultimate BOD
• Biodegradation rates
• Inhibitory concentration thresholds
• Nutrient ratio requirements (BOD:N:P)
• pH and temperature optima
• Screening of treatment alternatives
Scale 2
⚗️ Minireactors (Semi-continuous)
Laboratory-scale reactors operating under controlled semi-continuous conditions — the bridge between batch screening and full pilot plants. Allows determination of kinetic parameters and system performance under realistic operating regimes.
Typical determinations:
• HRT and SRT optimization
• Sludge yield coefficients
• Nitrification/denitrification kinetics
• Power density requirements
• Response to load variations
• Comparison of multiple configurations
Scale 3
🏭 Pilot Plants (Continuous)
Continuous laboratory-scale reactors extending over longer periods and testing several different scenarios. Maximum confidence in design parameters before full-scale construction. Regulatory acceptance for complex or sensitive applications.
Typical determinations:
• Full design parameter set
• Long-term stability and resilience
• Acclimatization periods
• Effluent quality under variable loads
• Sludge management parameters
• Regulatory-grade design basis
Aerobic and anaerobic treatability — both modes covered
💨 Aerobic Treatability
Activated sludge · SBR · MBR · aerated lagoons
Aerobic treatability studies determine the feasibility and optimal parameters for biological oxidation of organic matter and nutrients under oxygen-rich conditions.
• BOD removal efficiency vs. HRT
• Oxygen uptake rate (OUR) and SOUR
• Nitrification kinetics and inhibition
• Denitrification potential
• MLSS, SVI, sludge settleability
• Nutrient (N, P) requirements
• Toxicity and inhibition thresholds
🔄 Anaerobic Treatability
UASB · CSTR · anaerobic lagoons · biogas production
Anaerobic treatability studies — including Biochemical Methane Potential (BMP) — determine the feasibility of methane production and COD removal under oxygen-free conditions.
• Biochemical Methane Potential (BMP)
• Specific methanogenic activity (SMA)
• COD removal efficiency
• VFA accumulation and toxicity
• Ammonia inhibition thresholds
• OLR and HRT optimization
• Biogas composition (CH₄, CO₂, H₂S)
Key design parameters determined through treatability testing
The numbers that determine whether your plant will work
HRT
Hydraulic Retention Time
The minimum time effluent must remain in each treatment unit for the required removal to occur.
SRT / θc
Solids Retention Time
The average time a microorganism remains in the system. Determines biomass concentration and the types of organisms that can establish.
OUR / OTR
Oxygen Uptake / Transfer Rate
The actual oxygen demand of the mixed liquor — the basis for correct aeration system sizing.
F/M
Food-to-Microorganism Ratio
The ratio of substrate to biomass — determines system performance, sludge production and settling characteristics.
BOD:N:P
Nutrient Ratio
The optimal carbon to nitrogen to phosphorus ratio required to support biomass growth without nutrient limitation.
IC₅₀
Inhibitory Concentration
The concentration of specific compounds at which 50% inhibition of biological activity occurs — sets the maximum allowable loading.
OLR
Organic Loading Rate
The maximum organic load per unit volume per day that the system can handle without performance collapse.
Y / kd
Yield / Decay Coefficients
Biomass production per unit of substrate removed and endogenous decay rate — required for accurate sludge production calculations.
When treatability testing is essential
🏗️
New plant design
Before investing in construction of any biological treatment system — whether aerobic or anaerobic — treatability data must define the design basis. No reliable design can be produced without it.
🔄
Process changes
Any significant change in industrial process that alters effluent composition — new raw materials, products, cleaning chemicals — may require re-evaluation of treatability to maintain plant performance.
📉
Plant underperformance
When an existing plant is not meeting effluent quality targets, treatability testing provides the diagnostic data needed to identify the root cause and design corrective interventions.
📈
Capacity expansion
Before increasing hydraulic or organic loading to an existing system — verify that the biological community can handle the new conditions and identify any bottlenecks in advance.
⚖️
Regulatory compliance
Regulatory submissions for new discharge permits or EIA approvals frequently require treatability data demonstrating that the proposed system will achieve the required effluent quality.
🧩
Complex or novel effluents
Effluents containing inhibitory compounds, pharmaceutical residues, surfactants, or unusual industrial chemicals require specific treatability investigation — generic parameters simply do not apply.
Industries we serve
🌾
Agro-industry
Oilseed · grain · sugar · dairy
🥩
Food & Beverage
Slaughterhouses · breweries · soft drinks
🧪
Chemical industry
Surfactants · solvents · specialty chemicals
💊
Pharmaceutical
APIs · fermentation · cleaning effluents
📄
Pulp & Paper
High COD · colour · suspended solids
Petrochemical
BTEX · phenols · sulphur compounds
🔩
Metal finishing
Heavy metals · cyanide · chelating agents
🗑️
Landfill leachate
Recalcitrant COD · ammonia · metals
Why Biogroup for treatability testing
🔬
Own certified laboratory
Full analytical capability in-house — BOD, COD, TSS, nutrients, metals, VOCs and more. No outsourcing of analytical work, full control of data quality and turnaround time.
🧑‍🔬
Subject matter experts
Experienced environmental engineers and microbiologists who understand both the laboratory science and the full-scale design implications of treatability data.
📊
Design-oriented reports
Our treatability reports provide not just the data but the design parameters and recommendations needed to translate laboratory results into full-scale plant design.
🔗
Integrated services
Treatability testing seamlessly integrated with water and wastewater analysis, ecotoxicity testing, biogas potential assessment and remediation engineering — all under one roof.
Related services
Batch Tests · Minireactors · Pilot Plants · Aerobic & Anaerobic
Designing a treatment plant? Start with the data.
Biogroup has vast experience in treatability testing and subject matter experts who can help you solve treatment plant design and operational problems — before you invest in construction.
Request treatability testing →
📞 +54 341 425-6431 ✉ biogroup@biogroup.com.ar 📍 3 de febrero 920 · Rosario, Argentina Mon–Fri 08:00–17:00